![]() Only the nucleotide complementary to the template nucleotide at that position is added to the new strand.ĭNA polymerase contains a groove that allows it to bind to a single-stranded template DNA and travel one nucleotide at at time. The template strand specifies which of the four DNA nucleotides (A, T, C, or G) is added at each position along the new chain. ![]() The DNA fragments are joined by DNA ligase (not shown).ĭuring elongation, an enzyme called DNA polymerase adds DNA nucleotides to the 3′ end of the newly synthesized polynucleotide strand. On the leading strand, only a single RNA primer is needed, and DNA is synthesized continuously, whereas on the lagging strand, DNA is synthesized in short stretches, each of which must start with its own RNA primer. An RNA primer is synthesized by primase and is elongated by the DNA polymerase. \( \newcommand\): Replication Fork Formation: A replication fork is formed by the opening of the origin of replication helicase separates the DNA strands. ![]()
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